Anti-Human PEDF
- -
- -
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human PEDF (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831562 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human PEDF this polyclonal antibody can be used at a concentration of 0.2-2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human PEDF is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P232. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Pigment Epithelium Derived Factor (PEDF) recognizes Human PEDF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Pigment epithelium derived factor (PEDF) is non-inhibitory serpin with neurotrophic and anti-angiogenic functions.1 It protects immature but not mature granule cells against both natural and induced apoptosis.2 PEDF plays an important role in regulation of glial function and proliferation in the central nervous system.3 PEDF also plays important roles in the survival and maintenance of spinal motor neurons in their neuroprotection against acquired insults in postnatal life.4 It is suggested that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness.5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Zhang, SS. et al. (2005) J Struct Biol. 151:130 2. Schwartz, JP. et al. (1998) J Neurosci Res. 53:7 3. Schwartz, JP. et al. (1997) J Neurosci Res. 49:710 4. Kuncl, RW. et al. (1999) J Neuropathol Exp Neurol. 58:719 5. Bouck, NP. et al. (1999) Science 285:245 Technical ProtocolsIHC FFPE  Certificate of Analysis |
Related Products
- -
- -
Prod No. | Description |
|---|---|
P266 | |
P232 | |
S587 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
