Anti-Human PEDF – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen E. coli-Derived Recombinant Human Serpin F1 (aa 44 -418) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831796 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human PEDF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human PEDF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P232. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Pigment Epithelium Derived Factor (PEDF) recognizes Human PEDF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Pigment epithelium derived factor (PEDF) is non-inhibitory serpin with neurotrophic and anti-angiogenic functions.1 It protects immature but not mature granule cells against both natural and induced apoptosis.2 PEDF plays an important role in regulation of glial function and proliferation in the central nervous system.3 PEDF also plays important roles in the survival and maintenance of spinal motor neurons in their neuroprotection against acquired insults in postnatal life.4 It is suggested that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness.5 PubMed NCBI Gene Bank ID References & Citations1. Zhang, SS. et al. (2005) J Struct Biol. 151: 130 2. Schwartz, JP. et al. (1998) J Neurosci Res. 53: 7 3. Schwartz, JP. et al. (1997) J Neurosci Res. 49: 710 4. Kuncl, RW. et al. (1999) J Neuropathol Exp Neurol. 58: 719 5. Bouck, NP. et al. (1999) Science 285: 245 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
