Anti-Human PIKE (CT)

PN:P222

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human PIKE recognizes an epitope near the C-terminus of Human and Mouse PIKE. This polyclonal antibody was purified using affinity chromatography.

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity (1). PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin γ 1 (reviewed in 2). The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis.³ Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes.⁴ In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

1. Ye, K. et al. (2000) Cell 103:919
2. Jackson, TR. et al. (2000) Trends Biochem. Sci. 25:489
3. Rong, R. et al. (2003) Nat. Neurosci. 6:1153
4. Ahn, JY. et al. (2004) J. Biol. Chem. 279:16441