Anti-Human Pleiotrophin – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human PTN (Accession # Q6ICQ5) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831585 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human PTN this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human PTN is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P119. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Pleiotrophin (PTN) recognizes Human PTN. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), also known as PTN is a growth, differentiation inducing cytokine,1 and neurite extension promoting polypeptide which is highly expressed in brain and in tissues derived from mesenchyme.2 PTN is produced by many different human cancers and stimulates tumor blood vessel formation when it is expressed in malignant cancer cells.3 It is expressed in many types of cancer, such as gliomas, melanomas, meningiomas, neuroblastomas, choriocarcinomas, leukemias and cancers of pancreas, prostate, stomach, colon, breast, ovaries and lungs.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Lee, RJ. et al. (2005) Biochem Biophys Res Commun. 332: 1146 2. Deuel, TF. et al. (1992) Biochem Biophys Res Commun. 184: 427 3. Berenson, JR. et al. (2009) Blood 113: 1992 4. Papadimitriou, E. et al. (2007) Recent Patents Anticancer Drug Discov. 2: 175 Technical Protocols Certificate of Analysis |
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