Anti-Human/Primate BMP-9-Biotin

Anti-Human/Primate BMP-9-Biotin

Product No.: B647


Target BMP-9 Product Type Polyclonal Antibody Alternate Names Bone Morphogenetic Protein-9, GDF-2 Applications ELISA Det , WB


Select Product Size 50 µg — $590.00	Qty Max: Min: 1 Step: 1 $590.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human BMP-9 (Accession # Q9UK05) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828304 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human BMP-9 this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human BMP-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B624. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Bone Morphogenetic Protein 9 (BMP-9) recognizes Human BMP-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Bone morphogenetic protein (BMP-9) is also known as growth differentiation factor 2 (GDF2) is a protein that in humans encoded by the GDF2 gene. GDF2 belongs to the transforming growth factor beta (TGF-β) superfamily. BMP9 has a role in inducing and maintaining the ability of embryonal and adult tissues. Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP9 and remains associated with the mature peptide after proteolytic cleavage. Another suggested role of BMP-9 could be a potent inducer of hepcidin in liver cells and could regulate iron metabolism. Mature human and mouse BMP-9 share 96% amino acid sequence identity. PubMed BMP-9 NCBI Gene Bank ID 2658 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ELISA Det Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human BMP-9 (Accession # Q9UK05)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828304

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human BMP-9 this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human BMP-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B624.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Bone Morphogenetic Protein 9 (BMP-9) recognizes Human BMP-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Bone morphogenetic protein (BMP-9) is also known as growth differentiation factor 2 (GDF2) is a protein that in humans encoded by the GDF2 gene. GDF2 belongs to the transforming growth factor beta (TGF-β) superfamily. BMP9 has a role in inducing and maintaining the ability of embryonal and adult tissues. Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP9 and remains associated with the mature peptide after proteolytic cleavage. Another suggested role of BMP-9 could be a potent inducer of hepcidin in liver cells and could regulate iron metabolism. Mature human and mouse BMP-9 share 96% amino acid sequence identity.

PubMed

BMP-9

NCBI Gene Bank ID

2658

UniProt.org

More Information on Uniprot.org