Anti-Human SCF (Clone 13302)
Anti-Human SCF (Clone 13302)
Product No.: S499
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Clone 13302 Target SCF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Stem Cell Factor, c-kit Isotype IgG1 Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human SCF (Accession # P21583) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831761 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:S113 at a concentration of approximately 0.1 µg/ml. A suggested standard for this assay is PN:S109. Western Blotting: To detect Human SCF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human SCF is 100 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:S109. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Stem Cell Factor (SCF) (Clone 13302) recognizes an epitope on Human SCF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6 PubMed References & Citations1. Toppari, J. et al. (1999) Endocrin 140: 1492 2. Adamson, JW. et al. (1991) PNAS 88: 7420 3. Hervé, L. et al. (2005) J of Endocrin. 186: 131 4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74. 6. Phan, SH. et al. (2009) AM J Pathol. 174: 390 Technical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
