Anti-Human SDF-1β

Purified Recombinant Human SDF-1 β (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 10 ng/mL Recombinant Human/Feline CXCL12/SDF‑1 beta.

Goat Anti-Human Stromal Cell-Derived Factor-1 Beta (SDF-1 β) recognizes Human SDF-1 β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

SDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.¹ SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.²

1. Muller, HW. et al. (2000) Eur J Neurosci. 12:1857
2. Nagai, Y. et al. (1998) Proceedings of National Acad Sci USA 95:6331