Anti-Human Serpin E1 (Clone 242816)

Anti-Human Serpin E1 (Clone 242816)

Product No.: S606


Clone 242816 Target Serpin E1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Plasminogen Activator Inhibitor-1, Serpin E1, PAI-1 Isotype IgG1 Applications ELISA Cap , N , WB


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Data


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human Serpin E1 (Accession # P05121) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831806 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. Western Blotting: To detect Human Serpin E1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Serpin E1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P301. Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, #1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, #1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 0.3 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Serpin E1 (Clone 242816) recognizes an epitope on Human Serpin E1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed Serpin E1 NCBI Gene Bank ID 5054 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human Serpin E1 (Accession # P05121)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831806

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml.
Western Blotting: To detect Human Serpin E1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Serpin E1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P301.

Additional Applications Reported In Literature ?

Neutralization: Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, #1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, #1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 0.3 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Serpin E1 (Clone 242816) recognizes an epitope on Human Serpin E1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

Serpin E1

NCBI Gene Bank ID

5054

UniProt.org

Information on Uniprot.org