Anti-Human Siglec-2
Anti-Human Siglec-2
Product No.: S605
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Clone 219934 Target Siglec-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD22 Isotype IgG1 Applications CyTOF® , FC , IHC FFPE |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human Siglec-2 (Accession # CAA42006) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831805 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Siglec-2. A suggested method would be to stain cells expressing Siglec-2 with 25 µg per 1-2.5 x 106 cells in a total staining volume of 200 µl followed by PN:M1189. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Siglec-2 (Clone 219934) recognizes an epitope on Human Siglec-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed NCBI Gene Bank ID UniProt.org References & CitationsTechnical ProtocolsCyTOF®  IHC FFPE Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
