Anti-Human TCR Vα24-Jα18 (iNKT cell)- Purified in vivo GOLD™ Functional Grade
Anti-Human TCR Vα24-Jα18 (iNKT cell)- Purified in vivo GOLD™ Functional Grade
Product No.: T705
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Clone 6B11 Target TCR Vα24-Jα18 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT Isotype Mouse IgG1 κ Applications CyTOF® , FC , in vivo |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2894394 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TCR Vα24-Jα18 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? CyTOF® Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 6B11 recognizes the invariant CDR3 region of the human Vα24-Jα18 TCR. Background TCR Vα24-Jα18 antibody, 6B11, recognizes the CDR3 region of the V alpha 24 and J alpha 18 TCR alpha locus (Vα24-Jα18). The Vα24-Jα18 semi-invariant TCR is expressed on a highly conserved T cell subset, named invariant natural killer T (iNKT cells), which also express NK cell-associated molecules. The Vα24-Jα18 TCR recognizes lipid antigens, such as α-galactosylceramide (α-GalCer), presented by the MHC class I-like molecule CD1d1. Following activation, iNKT cells rapidly proliferate and produce large amounts of IL-4, IFNγ, and cytolytic mediators2,3. iNKT cells have both inflammatory and immunoregulatory functions and are implicated in anti-tumor immunity, protection against infections, suppression of autoimmunity, and the development of inflammatory diseases4.
Antigen Distribution The Vα24-Jα18 TCR is expressed on iNKT cells. Ligand/Receptor Glycolipid loaded CD1d NCBI Gene Bank ID Research Area Immunology References & Citations1. Van Kaer L., et al. (2004) Nat Rev Immunol. 4(3):231-7 2. Teyton L., et al. (2006) Annu Rev Immunol. 25:297–336 3. Kronenberg M. (2004) Annu Rev Immunol. 23:877–900 4. Van Kaer L & Wu L. (2018) Front Immunol. 9:519 Technical ProtocolsCyTOF®   Certificate of Analysis |
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Formats Available
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Prod No. | Description |
|---|---|
T705 | |
T1706 | |
T1709 | |
T1705 | |
T1707 | |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
