Anti-Human Thrombomodulin (CD141)

Anti-Human Thrombomodulin (CD141) – Polyclonal Antibody

Product No.: T547


Clone 501733 Target Thrombomodulin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD141, BDCA-3, TM Isotype IgG1 Applications CyTOF® , ELISA Cap , FC , IHC FFPE


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human THBD (Accession # P07204 (Val473)) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832027 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing THBD. A suggested method would be to stain cells expressing THBD with 25 µg per 1-2.5 x 10⁵ cells in a total staining volume of 200 µl followed by PN:M1189. ELISA Capture (Matched Antibody Pair): 2-8 µg/mL Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8-25 µg/mL. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Thrombomodulin (THBD) (Clone 501733) recognizes an epitope on Human THBD. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed Thrombomodulin NCBI Gene Bank ID 7056 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human THBD (Accession # P07204 (Val473))

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2832027

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing THBD. A suggested method would be to stain cells expressing THBD with 25 µg per 1-2.5 x 10⁵ cells in a total staining volume of 200 µl followed by PN:M1189.
ELISA Capture (Matched Antibody Pair): 2-8 µg/mL

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8-25 µg/mL. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Thrombomodulin (THBD) (Clone 501733) recognizes an epitope on Human THBD. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

Thrombomodulin

NCBI Gene Bank ID

7056

UniProt.org

Information on Uniprot.org