Anti-Human Tie-1 (Clone 88016)
Anti-Human Tie-1 (Clone 88016)
Product No.: T447
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Clone 88016 Target Tie-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tyrosine Kinase With Immunoglobulin-Like and EGF-Like Domains 1, JTK14, TIE Isotype IgG1 Applications ICC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human Tie-1 Extracellular Domain (Accession # P35590) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831947 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human Tie-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Tie-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Tie-1 (Clone 88016) recognizes an epitope on Human Tie-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie-1) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2Tie-1 also have a role in the regulatory pathways of early hematopoiesis and angiogenesis.3 It is upregulated in diseases such as atherosclerosis and rheumatoid arthritis.4 PubMed References & Citations1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272 2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704 3. Albita, M. et al. (2002) Cancer 94: 1517 4. Chan, B. and Sukhatme, VP. (2009) FEBS Lett. 583(6): 1023–1028. Technical ProtocolsICC IHC FFPE  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
