Anti-Human Tie-1
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human Tie 1 Extracellular Domain Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831969 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Tie 1. A suggested method would be to stain cells expressing Tie 1 with 0.50 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:G641. Western Blotting: To detect Human Tie 1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rhTie 1 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Tie 1 recognizes Human Tie 1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie-1) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2Tie-1 also have a role in the regulatory pathways of early hematopoiesis and angiogenesis.3 It is upregulated in diseases such as atherosclerosis and rheumatoid arthritis.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272 2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704 3. Albita, M. et al. (2002) Cancer 94: 1517 4. Sukhatme, VP. et al. (2009) FEBS Lett. Technical ProtocolsCyTOF®  IHC FFPE  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
