Anti-Human Tie-2 (Clone 83711)

Purified Recombinant Human Tie-2 (Accession # AAA61139)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Mouse Anti-Human Tie-2 recognizes an epitope on Human Tie-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.¹ They are expressed almost exclusively in endothelial cells.² Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.³ Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.

1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272
2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704
3. Boulton, ME. et al. (2008) Invest Ophthalmol Vis Sci 49: 2163