Anti-Human TIMP-2

Anti-Human TIMP-2

Product No.: T539


Target TIMP-2 Product Type Polyclonal Antibody Alternate Names Tissue Inhibitor of Metalloproteinase 2, CSC-21K Applications IHC FFPE , N , WB


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Data


Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human TIMP-2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832019 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human TIMP-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human TIMP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T531. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH₂ (10 µM). The Neutralization Dose (ND₅₀) is typically 2.6 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Human TIMP-2 (TIMP-2) recognizes Human TIMP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed TIMP-2 NCBI Gene Bank ID 7077 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human TIMP-2 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2832019

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human TIMP-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human TIMP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T531.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH₂ (10 µM). The Neutralization Dose (ND₅₀) is typically 2.6 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human TIMP-2 (TIMP-2) recognizes Human TIMP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

TIMP-2

NCBI Gene Bank ID

7077

UniProt.org

More Information on Uniprot.org