Anti-Human TNF-α (Clone 1825)

Purified Recombinant Human TNF-α (Accession # P01375)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human TNF-α bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TNF-α (at 0.25 ng/ml) is 0.02 - 0.04 µg/ml.

Clone 1825 recognizes an epitope on human TNF-α.

TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.

1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996