Anti-Human CD120b (TNFR2)

Anti-Human CD120b (TNFR2)

Product No.: T457


Clone 22210 Target TNFR2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor Receptor II, TNFRSF1B, p75, CD120b, TBPII, TNF-R75, TNFBR, TNFR2, TNFR80, p75TNFR Isotype IgG1 Applications ELISA Cap , N , WB


Select Product Size 25 µg — $290.00 100 µg — $510.00 500 µg — $0	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TNF RII (Accession # P20333) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831957 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T240 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T408. Western Blotting: To detect Human TNF RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNF RII is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T408. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human TNF RII bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TNF RII (at 0.3 ng/ml) is 0.5 - 1.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Tumor Necrosis Factor Receptor Type II (TNF RII) (Clone 22210) recognizes an epitope on Human TNF RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor receptor II (TNF-RII) is present on most cell types¹ and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.² PubMed TNFR2 NCBI Gene Bank ID 7133 UniProt.org Information on Uniprot.org References & Citations 1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217. 2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607. View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human TNF RII (Accession # P20333)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831957

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T240 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T408.
Western Blotting: To detect Human TNF RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNF RII is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T408.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human TNF RII bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TNF RII (at 0.3 ng/ml) is 0.5 - 1.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Tumor Necrosis Factor Receptor Type II (TNF RII) (Clone 22210) recognizes an epitope on Human TNF RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Tumor necrosis factor receptor II (TNF-RII) is present on most cell types¹ and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.²

PubMed

TNFR2

NCBI Gene Bank ID

7133

UniProt.org

Information on Uniprot.org