Anti-Human TRAIL R3 (Clone 90903)

NS0-Derived Recombinant Human TRAIL Receptor 3

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human TRAIL R3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TRAIL R3 (at 20 ng/ml) is 0.02 - 0.08 µg/ml.

Mouse Anti-Human TRAIL Receptor 3 (TRAIL R3) (Clone 90903) recognizes an epitope on Human TRAIL R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody displayed approximately 20% - 40% cross-reactivity with rhTRAIL RI and negligible cross-reactivity with rhTRAIL R2 and rhTRAIL R4.

TNF-related apoptosis-inducing ligand receptor 3 (TRAIL R3) is an antagonist decoy receptor¹ that can inhibit TRAIL-induced apoptosis.² TRAIL R3 is a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand.³

1. Fornace Jr, AJ. et al. (1999) Oncogene 18: 4153
2. Büchler, MW. et al. (2001) Anticancer Res. 21: 3153
3. Smith, CA. et al. (1997) J Exp Med. 186: 1165