Anti-Human TROP-2 (Clone 77220)
Anti-Human TROP-2 (Clone 77220)
Product No.: T454
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Clone 77220 Target TROP-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), EGP-1, GA733, GA733-1, M1S1, T16, GP50 Isotype IgG2a Applications CyTOF® , FC , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human TROP-2 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831954 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human TROP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhTROP-2 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human TROP-2 (Clone 77220) recognizes an epitope on Human TROP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhVCAM-1 and rhICAM-1. Background Trop-2 is a cell surface glycoprotein1 that is highly expressed in a wide variety of epithelial cancers. There is little or no expression of Trop-2 in adult somatic tissue.2 Trop-2 is a novel calcium signal transducer3 involved in the regulation of cell- cell adhesion.4 It is an oncogene that has potential as a therapeutic target.2 It is a possible candidate gene for diagnosis and molecular target therapy of colorectal cancer.5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Colnaghi, MI. et al. (1992) Hybridoma 11: 539 2. Michel, L. et al. (2008) Mol Cancer Ther. 7: 280 3. Alberti, S. et al. (1998) Int J Cancer 75: 324 4. Piantelli, M. et al. (2007) J Clin Oncol. 25: 10510 5. Mori, M. et al. (2006) clin Cancer Res. 12: 3057 Technical ProtocolsCyTOF®   Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
