Anti-Human VE-Cadherin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human VE-Cadherin Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832138 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing VE-Cadherin. A suggested method would be to stain cells expressing VE-Cadherin with 1 µg per 1.0-2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:G641. Western Blotting: To detect Human VE-Cadherin this polyclonal antibody can be used at a concentration of 0.25 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human VE-Cadherin is 5 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunocytochemistry: This antibody has been tested on human endothelial cells (HUVEC) and can be used with the appropriate secondary reagents at the concentration of 5-10 μg/mL. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with 0.1% Triton X-100, 10% normal donkey serum of PBS and 1% BSA at room temperature for 45 minutes. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Vascular Endothelial Cadherin (VE-Cadherin) recognizes Human VE-Cadherin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Vascular endothelial-cadherin (VE-Cadherin) also known as cadherin-5 is an endothelial specific, homophilic adhesion molecule located at the lateral cell surface.1 VE-Cadherin is crucial for the proper assembly of vascular structures and the maintenance of vascular integrity.2 It plays a pivotal role in the formation, maturation and remodeling of the vascular wall.3 VE-cadherin is a positive and endothelial cell-specific regulator of transforming growth factor-beta (TGF-beta) signaling. Therefore, the reduction or inactivation of VE-cadherin may contribute to progression of diseases where TGF-beta signaling is impaired.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Vestweber, D. et al. (1997) J Cell Sci. 110: 583 2. Lambert, O. et al. (2008) Biochem Soc Trans. 36: 189 3. Gavard, J. et al. (2009) FEBS Lett. 583: 1 4. Dejana, E. et al. (2008) EMBO J. 27: 993 Technical ProtocolsCyTOF®  ICC  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
