Anti-Human X Box Binding Protein 1 (NT) (XBP-1)

PN:X110

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human X Box Binding Protein 1 (XBP-1) recognizes an epitope near the N-terminus of Human XBP-1. This polyclonal antibody was purified using affinity chromatography.

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.^3,4

1. Yoshida, H. et al. (2001) Cell 107:881
2. Calfon, M. et al. (2002) Nature 415:92
3. Haze, K. et al. (1999) Mol. Cell. Biol. 10:3787
4. Little, E. et al. (1994) Crit. Rev. Eukaryot. Gene Expr. 4:1