Anti-Ly49C (Clone 4LO3311) – FITC

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Blue Laser (490 nm)

4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1). 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.

Ly49 receptors are homodimeric type II C-type lectin-like membrane glycoproteins encoded by a family of highly polymorphic genes in the mouse natural killer (NK) gene complex¹. Ly49 receptors recognize class I major histocompatibility complex-I (MHC-1) and MHC-1 like proteins. Ly49C is an inhibitory member of the Ly-49 superfamily. Inhibitory Ly49 receptors are involved in NK cell education¹ and play a role in controlling viral infection². Additionally, Ly49C decreases the activation threshold of NK cells by inhibiting H2-K^{b 3}. Ly49C is expressed on NK, uterine NK, NKT, and CD8⁺ Tregs cells¹. Ly49C is closely related to Ly49F, Ly49I, and Ly49H³. 4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells⁴. Hybridoma lines were prepared by fusing immune spleen cells with non-secreting P3X63-Ag8.653 myeloma cells and subsequently screened for production of anti-NK antibodies. Isotype was determined by double-immunodiffusion analysis of hybridoma supernatants using rabbit monospecific anti-mouse immunoglobulins. 4LO3311 is of IgG3, κ isotype. 4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain⁵. 4LO3311 recognizes Ly-49C^BALB but not Ly-49A^B6, Ly-49A^BALB, B^BALB, D^B6, E^B6, F^B6, G^B6, G^BALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells⁴. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.

Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.

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