Anti-Mouse CCL17 (Clone 110904)
Anti-Mouse CCL17 (Clone 110904)
Product No.: C1435
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Clone 110904 Target CCL17 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TARK, ABCD-2, MGC138271, SCYA17, TARC Isotype IgG2a Applications ELISA Cap , N |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CCL17 (Accession No.: Q9WUZ6) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828496 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:C1510 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is PN:T397. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CCL17 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL17 (at 20 ng/ml) is 0.75 - 30 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse CCL17 (Clone 110904) recognizes an epitope on Mouse CCL17. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).3 It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.4 PubMed Research Area Chemokine References & Citations1. Yoshie, O. et al. (1996) J Biol Chem. 271: 21514 2. Yoshie, O. et al. (1997) J Biol Chem. 272: 15036 3. Kamatani, N. et al. (2003) J Rheumatology 30: 2369 4. Kunihiko, K. et al. (2006) Eur J Immunol. 36: 2116 Technical Protocols N Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
