Anti-Mouse CCL25 (Clone 89827)

Product No.: C1429


Clone 89827 Target CCL25 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYA25, TECK, Ckb15, Chemokine (C-C Motif) Ligand 25 Isotype IgG2a Applications ELISA Cap , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CCL25 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828489 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T491 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T396. Western Blotting: To detect Mouse CCL25 this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL25 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T396. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:R1215. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse CCL25 (Clone 89827) recognizes an epitope on Mouse CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with other chemokines teste Background Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.¹ It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.² CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.³ PubMed CCL25 Research Area Chemokine References & Citations 1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301. 2. Agace, W. et al. (2006) J Immunol. 176:3642 3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380 View product citations for antibody C1429 on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse CCL25 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828489

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T491 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T396.
Western Blotting: To detect Mouse CCL25 this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL25 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T396.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:R1215. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CCL25 (Clone 89827) recognizes an epitope on Mouse CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with other chemokines teste

Background

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.¹ It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.² CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.³

PubMed

CCL25

Research Area

Chemokine

References & Citations

1. Zlotnik, A. et al. (1997) Immunity. 7(2):291-301.
2. Agace, W. et al. (2006) J Immunol. 176:3642
3. Teixidó, J. et al. (2007) J Leukoc Biol. 82:380

View product citations for antibody C1429 on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
C1428	Anti-Mouse CCL25 (Clone 89818)
C1399	Anti-Human CCL25 (Clone 52529)
C1429	Anti-Mouse CCL25 (Clone 89827)
T396	Recombinant Mouse TECK
T491	Anti-Mouse CCL25-Biotin
T185	Anti-Human CCL25-Biotin

Formats Available

Prod No.	Description
C1429	Anti-Mouse CCL25 (Clone 89827)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CCL25 (Clone 89827)