Anti-Mouse CCL7
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse MCP-3 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method State of Matter Solution Storage and Handling The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831361 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MCP-3 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-3 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M134. Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 7.5-45 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL7/MCP‑3/MARC. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse Monocyte Chemotactic Protein-3 (MCP-3) recognizes Mouse MCP-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.1 MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes. MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.2 MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.3,4,5 PubMed NCBI Gene Bank ID UniProt.org Research Area Chemokine References & Citations1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535 2. Parmentier, M. et al. (1999) Blood 94: 1899 3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20 4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260 5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171 Technical ProtocolsN  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
