Anti-Mouse CD105 (Endoglin) (Clone MJ7/18) – HRP

Inflamed mouse skin

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. <b> (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)</b>

This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze.

IHC^{2, 4, 7, 8, 9, 10},
IHC FF¹⁹
IF⁵,
IF Microscopy⁶,
IP⁷,
Cell Sep-Pos^{12, 15},
Agonist^{14, 16},
LCI^{17, 18}

Anti-CD105 antibody (clone MJ7/18) activity is directed against mouse CD105 (endoglin).

CD105 (endoglin) is a TGF-β superfamily co-receptor that promotes angiogenesis, is involved in endothelial integrin-mediated mural cell and leukocyte adhesion, antagonizes TGF-β mediated ERK activation, is essential to the immune response of macrophages, regulates trophoblast differentiation and invasion during pregnancy, promotes T-cell proliferation, and regulates differentiation and collagen expression in myofibroblasts¹. CD105 is dysregulated in the vasculature of multiple diseases including cancer, preeclampsia, and hereditary hemorrhagic telangiectasia. Additionally, increased soluble CD105 is associated with numerous cardiovascular pathologies and metabolic disorders with some CD105 polymorphisms being associated with increased risk of cardiovascular damage. CD105 is an effective marker of the tumor vasculature and is a target for early cancer diagnosis and antiangiogenic therapies.

CD105 is alternatively spliced, resulting in both long (L-endoglin) and short (S-endoglin) forms¹. The predominant isoform, L-endoglin, promotes the proliferation and migration of endothelial cells via enhanced ALK1-Smad1/5 signaling and is the presumed isoform when not specified in the literature. In contrast, S-endoglin enhances ALK5-Smad2/3 signaling. Endoglin has specific receptor-ligand interactions between type I and type II TGF-β superfamily receptors when binding TGF-β superfamily ligands. Mouse CD105 has three separate regions of similarity to TGF-β receptor III but does not contain the RGD tripeptide found in human CD105².

MJ7/18 was produced by immunizing rats with inflamed mouse skin and selecting for reactivity with endothelial cells^{2, 3}. MJ7/18 predominantly stains vascular endothelial cells and is a marker of mouse endothelium^{2, 4}.

TGF-ß1, TGF-ß3

1. Pawlak JB, Blobe GC. Dev Dyn. 251(1):137-163. 2022.
2. Ge AZ, Butcher EC. Gene. 138(1-2):201-206. 1994.
3. Berg EL, Goldstein LA, Jutila MA, et al. Immunol Rev. 108:5-18. 1989.
4. Hallmann R, Mayer DN, Berg EL, et al. Dev Dyn. 202(4):325-332. 1995.
5. Charbord P, Oostendorp R, Pang W, et al. Exp Hematol. 30(10):1202-1210. 2002.
6. Rivera LB, Brekken RA. J Cell Biol. 193(7):1305-1319. 2011.
7. Romero D, O'Neill C, Terzic A, et al. Cancer Res. 71(10):3482-3493. 2011.
8. Kruse A, Hallmann R, Butcher EC. Biol Reprod. 61(6):1393-1401. 1999.
9. Redaelli CA, Semela D, Carrick FE, et al. J Hepatol. 40(2):305-312. 2004.
10. Arguello AA, Fischer SJ, Schonborn JR, et al. Neuroscience. 159(3):1003-1010. 2009.
11. Izawa D, Tanaka T, Saito K, et al. Int Immunol. 11(12):1989-1998. 1999.
12. Lidington EA, Rao RM, Marelli-Berg FM, et al. Am J Physiol Cell Physiol. 282(1):C67-74. 2002.
13. Garton KJ, Gough PJ, Philalay J, et al. J Biol Chem. 278(39):37459-37464. 2003.
14. Kinderlerer AR, Pombo Gregoire I, Hamdulay SS, et al. Blood. 113(7):1598-1607. 2009.
15. Marelli-Berg FM, Peek E, Lidington EA, et al. J Immunol Methods. 244(1-2):205-215. 2000.
16. Ahmad SR, Lidington EA, Ohta R, et al. Immunology. 110(2):258-268. 2003.
17. Karmani L, Bouchat V, Bouzin C. Nanomedicine (Lond). 9(13):1923-1937. 2014.
18. Karmani L, Levêque P, Bouzin C, et al. Nucl Med Biol. 43(7):415-423. 2016.
19. Engelhardt B, Conley FK, Butcher EC. J Neuroimmunol. 51(2):199-208. 1994.