Anti-Mouse CD11b – Biotin

C57BL/10 splenocytes

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Next Day Ambient

ICC
IHC (Frozen)

Depletion
FA
IHC (Frozen)
IHC (Paraffin)
IP
IF Microscopy
For specific conjugates of this clone, review literature for suggested application details.

Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen

1. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539 2. Springer, T.A., et al. (1982) Immunol. Rev. 68:171 3. Ding, A., et al. (1987) J Exp Med. 165:733 4. Vignali, D. A. et al. (1990) J. Immunol. 144:4030 5. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320