Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo GOLD™ Functional Grade
Product No.: C2325
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Clone TM-β1 Target CD122 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-2Rβ, Interleukin 2 receptor β chain, IL-2/15Rb Isotype Rat IgG2b κ Applications B , Depletion , FC , in vivo , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Rat T-cell line expressing Mouse IL-2Rβ Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737464 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TM-β1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this TM-β1 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? B IP Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone TM-β1 recognizes an epitope on mouse CD122. Background CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor. Antigen Distribution CD122 is expressed on NK cells and at lower levels by T lymphocytes, B lymphocytes, monocytes, and macrophages. Ligand/Receptor IL-2, IL-15 Function Critical component of IL-2 and IL-15 signaling PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Burchill, MA. et al. (2007) J. Immunol. 178:280 2. Friedmann, MC. et al. (1996) Proc. Natl. Acad. Sci. (USA) 93:2077 3. Leonard, WJ. et al. (1987) Science 238:75 Technical ProtocolsB Depletion     Certificate of Analysis |
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Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
