Anti-Mouse CD172a -BX013 (P84); Alexa 488-RX013

Alexa Fluor™ 488 is in the bright green fluorescent channel with an excitation max of 490 nm and an emission max of 525 nm.

Mouse brain membrane protein

This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)

Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.

CODEX® barcoded antibody - Liquid; CODEX® reporter - Liquid

This PhenoCycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.

Clone P84 recognizes an epitope on mouse CD172a.

CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.

CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)¹. SIRPα is expressed predominantly in myeloid cells² - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells³. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])⁴, is expressed in most cell types⁵. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells^6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation^8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages¹¹. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics¹².

PhenoCycler-Fusion (CODEX)® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a PhenoCycler-Fusion (CODEX)® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

1. Fujioka, Y., et al. (1996) Mol. Cell. Biol. 16:6887
2. Adams, S., et al. (1998) J. Immunol. 161:1853
3. Chuang W, et al. (1990) Dev Biol. 137:219–232
4. Seiffert, M., et al. (1999) Blood 94:3633
5. Oldenborg P. A. (2013) ISRN Hematol. 2013:614619
6. Oldenborg, P. A., et al. (2000) Science 288:2051
7. Oldenborg, P. A., et al. (2001) J. Exp. Med. 193:855
8. Brooke, G. P., et al. (1998) Eur. J. Immunol. 28:1
9. Seiffert, M., et al. (2001) Blood 97:2741
10. Latour, S., et al. (2001) J. Immunol. 167:2547
11. Jaiswal S, et al. (2009) Cell. 138(2):271-85
12. Jalil AR, et al. (2020) Antib Ther. 3(2):80-94