Anti-Mouse CD172a – Purified in vivo GOLD™ Functional Grade

Mouse brain membrane protein

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone P84 recognizes an epitope on mouse CD172a.

CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)¹. SIRPα is expressed predominantly in myeloid cells² - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells³. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])⁴, is expressed in most cell types⁵. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells^6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation^8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages¹¹. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics¹².

CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.

CD47, SP-A, SP-D

Negative regulation of several biological processes

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2. Adams, S., et al. (1998) J. Immunol. 161:1853
3. Chuang W, et al. (1990) Dev Biol. 137:219–232
4. Seiffert, M., et al. (1999) Blood 94:3633
5. Oldenborg P. A. (2013) ISRN Hematol. 2013:614619
6. Oldenborg, P. A., et al. (2000) Science 288:2051
7. Oldenborg, P. A., et al. (2001) J. Exp. Med. 193:855
8. Brooke, G. P., et al. (1998) Eur. J. Immunol. 28:1
9. Seiffert, M., et al. (2001) Blood 97:2741
10. Latour, S., et al. (2001) J. Immunol. 167:2547
11. Jaiswal S, et al. (2009) Cell. 138(2):271-85
12. Jalil AR, et al. (2020) Antib Ther. 3(2):80-94