Anti-Mouse CD19 – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD19 – Purified in vivo PLATINUM™ Functional Grade
Product No.: C2849
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Clone 1D3 Target CD19 Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications B , Depletion , FC , IHC FF , in vivo , IP , PhenoCycler® , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen K562 cells expressing the extracellular domain of mouse CD19 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829604 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 1D3 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 1D3 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? PhenoCycler-Fusion (CODEX)® B Depletion IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 1D3 recognizes an epitope on mouse CD19. Background CD19 is a 95 kD transmembrane glycoprotein and member of the Ig superfamily. The antigen serves as an adaptor protein; drawing cytoplasmic signaling proteins to the membrane. It works via the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Because of its presence on all B cells, CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for the immunotherapy of lymphoproliferative disorders. Emerging studies indicate that CD19 plays an active role in fueling the growth of these cancers, most notably by stabilizing the concentrations of the MYC oncoprotein, making CD19 an attractive therapeutic target with respect to its downstream signaling. Antigen Distribution CD19 is expressed in the majority of Pro-B cells to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. Ligand/Receptor CD21, CD81, Leu-13. PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology References & CitationsTechnical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C2114 | |
C2115 | |
C2108 | |
C2821 | |
C2104 | |
C2105 | |
C2088 | |
C2107 | |
C2109 | |
C2110 | |
C2111 | |
C2113 | |
C2106 | |
C525 | |
C2117 | |
C2849 |
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