Anti-Mouse CD226 (DNAM-1) – Purified in vivo PLATINUM™ Functional Grade

Th1 polarized T cell clones

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1).

Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)¹. mCD226 is also essential for NK-mediated killing of immature dendritic cells² and acts as an important costimulatory molecule in Th1 cell activation and effector function³.

CD226 binds to PVR (CD155) via its N-terminal Ig-like domain² and with cell surface receptor NECTIN2 (CD112)¹. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding.

mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells³. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8⁺ T cells, subsets of naïve CD11b⁺ macrophages and NK cells, and is up-regulated on activated CD4⁺ cells^2,3. mCD226 is a marker for mature T cells².

mCD226 is expressed on the surface of differentiated Th1 cells, CD8⁺ T cells, subsets of naïve CD11b⁺ macrophages and NK cells, and activated CD4⁺ cells.

1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009.
3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005.
4. He Y, Peng H, Sun R, et al. J Autoimmun. 81:1-12. 2017.