Anti-Mouse CD226 – Purified in vivo GOLD™ Functional Grade

Murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

10E5 activity is directed against murine CD226 (DNAM-1; PTA-1).

Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T- lymphocyte and natural killer (NK) cells (Uniprot Q8K4F0)¹. mCD226 is also essential for NK- mediated killing of immature dendritic cells ² and acts as an important costimulatory molecule in Th1 cell activation and effector function³.

CD226 binds to PVR (CD155) via its N-terminal Ig-like domain² and with cell surface receptor NECTIN2 (CD112)¹. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding.

mCD226 is a marker for mature T cells² and is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells³. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization.

10E5 was generated by immunizing Lewis and Lou/M rats with established murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice³. The resulting panel was screened on polarized Th1 and Th2 T cell clones and cell lines to find an antibody that selectively recognizes only Th1 clones. 10E5 specifically recognizes CD226 on the surface of Th1 cells. Additionally, 10E5 inhibits Ag-specific T cell expansion, reducing the expansion of PLP 139-151-specific Th1 cells producing IFN-γ. In vivo treatment with anti- CD226 clone 10E5 inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease.

mCD226 is expressed mainly on T cells, including on the surface of differentiated Th1 cells, almost all naïve CD8⁺ T cells, activated CD4⁺ cells, about 40% of unactivated CD4⁺ T cells as well as on subsets of primary CD11b⁺ macrophages, CD11c⁺ dendritic cells and NK1.1 and DX5 NK cells.

CD112 and CD155

1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009.
3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005.