Anti-Mouse CD25 – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD25 – Purified in vivo PLATINUM™ Functional Grade
Product No.: C2845
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Clone PC61 Target CD25 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac, IL-2 Rα Isotype IgG1 λ Applications B , Depletion , FA , FC , IHC FF , in vivo , IP , PhenoCycler® , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Dilution Buffer Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829601 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? PhenoCycler-Fusion (CODEX)® B Depletion IHC (Frozen) IP FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone PC61 recognizes an epitope on mouse CD25. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Antigen Distribution CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. Ligand/Receptor IL-2 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Braley-Mullen, H. et al. (2018) Immunohorizons. 2(1): 54–66. PubMed 2. Leonard, WJ. et al. (2002) The EMBO Journal 21: 3051 3. Alt, FW. et al. (1995) Immnnity 3: 521 4. Greene, WC. et al. (1990) J Invest Dermatol. 94: 27S Technical ProtocolsB Depletion FA  IHC FF   PhenoCycler®  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C1924 | |
C1189 | |
C1193 | |
C1192 | |
C1191 | |
C1916 | |
C1917 | |
C1918 | |
C1920 | |
C1922 | |
C526 | |
C1194 | |
C1190 | |
C2845 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
