Anti-Mouse CD252 (OX40L) – DyLight® 650
Anti-Mouse CD252 (OX40L) – DyLight® 650
Product No.: C2345
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Clone RM134L Target OX 40 Ligand Formats AvailableView All Product Type Monoclonal Antibody Alternate Names OX 40 Ligand, OX40L, CD134L, TNFSF4 Isotype IgG2b Applications FC |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Rat NRK-52E cell line transfected with mouse OX-40L gene Product Concentration 0.2 mg/ml Formulation This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Product Preparation This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Followed by purification this antibody was conjugated to DyLight 650 using optimal conditions. Storage and Handling This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Red Laser (652 nm) RRIDAB_2829437 Applications and Recommended Usage? Quality Tested by Leinco For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each lot of this antibody is quality control tested. Additional Applications Reported In Literature ? Additional reported applications (for the relevant formats) B:The RM134L antibody can block the costimulatory activity of OX40L. IHC-FFPE Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Conjugation DetailsReporter Molecule DyLight®650 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. DescriptionDescriptionSpecificity Rat Anti-Mouse OX 40 Ligand (CD252) (Clone RM134L) recognizes an epitope on Mouse CD252. Background CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory. Antigen Distribution Activated B cells, antigen presenting cells Ligand/Receptor OX40 (CD134) Function B-cell/T-cell interaction, costimulation NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology References & CitationsTechnical Protocols Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C2338 | |
C2339 | |
C2342 | |
C2345 | |
C2336 | |
C2146 | |
C2340 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
