Anti-Mouse ICOSL (CD275) (Clone HK5.3) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse ICOSL (CD275) (Clone HK5.3) – Purified in vivo GOLD™ Functional Grade
Product No.: C2369
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Clone HK5.3 Target B7-H2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ICOSLG, ICOS-L, B7RP-1, LICOS, B7 homolog 2, B7-H2, B7H2, B7h Isotype Rat IgG2a Applications B , FC , in vivo , N |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Transfected Cell Line for Mouse B7H2 Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829462 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. Additional Applications Reported In Literature ? Additional reported applications (for the relevant formats) include: Blocking ligand binding Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse ICOSL (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background ICOSL, also known as CD275 and B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. ICOSL is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function1. ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway2. The structural features of ICOSL are crucial for its costimulatory function. ICOSL constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein3. Antigen Distribution splenic B-cells, T-cells, dendritic cells and macrophages Ligand/Receptor ICOS Function Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Wiendl, H. et al. (2003) Brain 126:1026 2. Yoshinaga, SK. et al. (1999) Nature 402:827 3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920 Technical ProtocolsB   N Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
