Anti-Mouse ICOSL (CD275) (Clone HK5.3) – PE
Anti-Mouse ICOSL (CD275) (Clone HK5.3) – PE
Product No.: C2373
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Clone HK5.3 Target B7-H2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ICOSLG, ICOS-L, B7RP-1, LICOS, B7 homolog 2, B7-H2, B7H2, B7h Isotype IgG2a Applications FC |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Transfected Cell Line for Mouse B7H2 Product Concentration 0.2 mg/ml Formulation This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Product Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. Storage and Handling This R-PE antibody conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm) RRIDAB_2829466 Applications and Recommended Usage? Quality Tested by Leinco FC:For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.
Each lot of this antibody is quality control tested. Additional Applications Reported In Literature ? Additional reported applications (for the relevant formats) include: Blocking NOTE: The purified functional grade GOLD antibody is recommended for functional assays (Part No.: C2369). Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse ICOSL (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background ICOSL, also known as CD275 and B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. ICOSL is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function1. ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway2. The structural features of ICOSL are crucial for its costimulatory function. ICOSL constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein3. Ligand/Receptor ICOS Function Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Wiendl, H. et al. (2003) Brain 126:1026 2. Yoshinaga, SK. et al. (1999) Nature 402:827 3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
