Anti-Mouse CD278 (ICOS) (Clone 7E.17G9) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD278 (ICOS) (Clone 7E.17G9) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C2851
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Clone 7E.17G9 Target CD278 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Inducible Costimulator,ICOS Isotype Rat IgG2b Applications B , FA , FC , in vivo , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse ICOS cDNA and ICOS hexahistidine fusion protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829607 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 7E.17G9 recognizes an epitope on mouse CD278. Background CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases. Antigen Distribution ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages. Ligand/Receptor B7h/B7RP-1/GL-50 PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology References & Citations1. Flavell, R. A. et al. (2001) Nature 409:97
2. Bachmann, M. F. et al. (2000) J. Exp. Med. 192:53
3. Kroczek, R. A. et al. (1999) Nature 397:263
Technical ProtocolsB FA    Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
