Anti-Mouse CD28 (Clone 37.51) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD28 (Clone 37.51) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C1658
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Clone 37.51 Target CD28 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names MGC138290, Tp44 Isotype IgG Applications B , Costim , CyTOF® , FA , FC , IHC FF , in vivo , IP , WB |
Data
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Syrian Hamster Recommended Dilution Buffer Immunogen C57BL/6 mouse T-cell lymphoma EL-4 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 20 mM L-histidine and 150 mM NaCl buffer, pH 5.9-6.1 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Shipping Next Day 2-8°C RRIDAB_2828714 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? CyTOF® IHC (Frozen) B Costim IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 37.51 recognizes an epitope on mouse CD28. Background CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic. Antigen Distribution CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells. Ligand/Receptor CD80 (B7-1), CD86 (B7-2) NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology References & Citations1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed 2. Gross, J. A. et al. (1992) J. Immunol. 149:380 Technical ProtocolsB Costim CyTOF® FA  IHC FF    Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C1706 | |
C1707 | |
C1709 | |
C243 | |
C244 | |
C246 | |
C1701 | |
C1702 | |
C1703 | |
C1705 | |
C347 | |
C379 | |
C1658 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
