Anti-Mouse CD28 - PE - Leinco Technologies

Anti-Mouse CD28 – PE

Product No.: C246


Clone 37.51 Target CD28 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names MGC138290, Tp44 Isotype IgG Applications FC


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Data


Antibody Details Product Details Reactive Species Mouse Host Species Syrian Hamster Immunogen Purified Recombinant Mouse CD28 (>98%) Product Concentration 0.2 mg/ml Formulation This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This R-PE conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm) RRIDAB_2829544 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? B Cosim IP IHC (Frozen) For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Clone 37.51 recognizes an epitope on mouse CD28. Background CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic. Antigen Distribution CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells. Ligand/Receptor CD80 (B7-1), CD86 (B7-2) PubMed CD28 NCBI Gene Bank ID 12487 UniProt.org Information on Uniprot.org Research Area Costimulatory Molecules . Immunology References & Citations Gross, J. A. et al. (1992) J. Immunol. 149:380 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Syrian Hamster

Immunogen

Purified Recombinant Mouse CD28 (>98%)

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRIDAB_2829544

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates ?

B
Cosim
IP
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 37.51 recognizes an epitope on mouse CD28.

Background

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Distribution

CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

PubMed

CD28

NCBI Gene Bank ID

12487

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

Immunology

References & Citations

Gross, J. A. et al. (1992) J. Immunol. 149:380

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
C1706	Anti-Mouse CD28 – DyLight® 680
C1707	Anti-Mouse CD28 – DyLight® 755
C1709	Anti-Mouse CD28 – PerCP
C1702	Anti-Mouse CD28 – DyLight® 550
C1703	Anti-Mouse CD28 – DyLight® 594
C1705	Anti-Mouse CD28 – DyLight® 650
C2820	Anti-Human CD28 – Purified in vivo GOLD™ Functional Grade
C1658	Anti-Mouse CD28 (Clone 37.51) – Purified in vivo PLATINUM™ Functional Grade

Formats Available

Prod No.	Description
C1706	Anti-Mouse CD28 – DyLight® 680
C1707	Anti-Mouse CD28 – DyLight® 755
C1709	Anti-Mouse CD28 – PerCP
C243	Anti-Mouse CD28 (Clone 37.51) – Purified
C244	Anti-Mouse CD28 (Clone 37.51) – Biotin
C246	Anti-Mouse CD28 – PE
C1701	Anti-Mouse CD28 – DyLight® 488
C1702	Anti-Mouse CD28 – DyLight® 550
C1703	Anti-Mouse CD28 – DyLight® 594
C1705	Anti-Mouse CD28 – DyLight® 650
C347	Anti-Mouse CD28 – APC
C379	Anti-Mouse CD28 (Clone 37.51) – Purified in vivo GOLD™ Functional Grade
C1658	Anti-Mouse CD28 (Clone 37.51) – Purified in vivo PLATINUM™ Functional Grade

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CD28 – PE