Anti-Mouse CD3ε (Clone 145-2C11) F(ab’)2 fragment – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD3ε (Clone 145-2C11) F(ab’)2 fragment – Purified in vivo GOLD™ Functional Grade
Product No.: C1758-FAB2
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Clone 145-2C11 Target CD3ε Formats AvailableView All Product Type F(ab')2 Monoclonal Antibody Alternate Names CD3, T3 Isotype Hamster IgG1 Applications Depletion , in vivo |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Isotype Controls Recommended Dilution Buffer Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737455 Additional Applications Reported In Literature ? Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 145-2C11 recognizes an epitope on mouse CD3ε. Background Derived from the 145-2C11 monoclonal antibody, this product provides the F(ab’)2 fragment, targeting the 20 kDa transmembrane CD3ε protein, a crucial component of the TCR complex on T lymphocytes, NK-T cells, and thymocytes. Pepsin digestion removes the Fc region, ensuring minimal non-specific binding and preventing Fcγ receptor-mediated cross-linking. This F(ab’)2 fragment is designed for in vivo applications where the full 145-2C11 antibody's T cell activation can lead to adverse effects. It induces apoptosis in antigen-activated T cells by promoting sustained TCR expression and signaling, while Foxp3+ regulatory T cells remain unaffected. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Ligand/Receptor Peptide antigen/MHC-complex Function TCR signal transduction, T cell activation, antigen recognition PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1.) Abdulreda, M.H. et al. (2019) Diabetologia. 63, 237–1250 2.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5. Journal Link |
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