Anti-Mouse CD3ε (Clone 145-2C11) F(ab’)2 fragment – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD3ε (Clone 145-2C11) F(ab’)2 fragment – Purified in vivo GOLD™ Functional Grade

Product No.: C1758-FAB2

[product_table name="All Top" skus="C1758"]

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Clone
145-2C11
Target
CD3ε
Formats AvailableView All
Product Type
F(ab')2 Monoclonal Antibody
Alternate Names
CD3, T3
Isotype
Hamster IgG1
Applications
Depletion
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Recommended Dilution Buffer
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Additional Applications Reported In Literature ?
Depletion
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Background
Derived from the 145-2C11 monoclonal antibody, this product provides the F(ab’)2 fragment, targeting the 20 kDa transmembrane CD3ε protein, a crucial component of the TCR complex on T lymphocytes, NK-T cells, and thymocytes. Pepsin digestion removes the Fc region, ensuring minimal non-specific binding and preventing Fcγ receptor-mediated cross-linking. This F(ab’)2 fragment is designed for in vivo applications where the full 145-2C11 antibody's T cell activation can lead to adverse effects. It induces apoptosis in antigen-activated T cells by promoting sustained TCR expression and signaling, while Foxp3+ regulatory T cells remain unaffected.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Ligand/Receptor
Peptide antigen/MHC-complex
Function
TCR signal transduction, T cell activation, antigen recognition
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1.) Abdulreda, M.H. et al. (2019) Diabetologia. 63, 237–1250
2.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5. Journal Link
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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.