Anti-Mouse CD3ε (Clone 500A2) – Purified

Mouse T-cell receptor

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Next Day Ambient

IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 2.5 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
B

Clone 500A2 recognizes an epitope on mouse CD3ε.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.

Peptide antigen/MHC-complex.

Havran, W. M. et al. (1987) Nature 330:170
Allison, J. W. et al. (1988) The T-Cell Receptor 33