Anti-Mouse CD3 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Clone 17A2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.

γ/δ TCR-positive T-T hybridoma D1

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

Functional grade preclinical antibodies are manufactured in an animal free facility using <i>in vitro</i> cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

AB_2893570

Clone 17A2 recognizes an epitope on mouse CD3.

CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Peptide antigen/MHC-complex

Antigen recognition, TCR signal transduction, T cell activation

1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed
2. Mysliwietz, J. et al. (1992) Blood 80: 2661-2667