Anti-Mouse CD3ε (Clone 500A2) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD3ε (Clone 500A2) – Purified in vivo GOLD™ Functional Grade
Product No.: C219
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Clone 500A2 Target CD3ε Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD3ε, T3, CD3 Isotype IgG2a κ Applications B , FC , IHC FF , in vivo |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Syrian Hamster Recommended Isotype Controls Syrian Hamster IgG2a κ Recommended Dilution Buffer Immunogen Mouse T-cell receptor Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737460 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 2.5 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 500A2 recognizes an epitope on mouse CD3ε. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Ligand/Receptor Peptide antigen/MHC-complex. Function TCR signal transduction, T cell activation and antigen recognition. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Signal Transduction References & Citations1. Havran, WM. et al. (1987) Nature 330:170 2. Allison, JW. et al. (1988) The T-Cell Receptor 33 Technical ProtocolsB  IHC FF  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
