Anti-Mouse CD32/CD16 – Purified (Fc Block Antibody)

Sorted pre-B cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Leinco antibodies are manufactured in an animal-free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

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IP
B Rat Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ may be used for blocking Fc receptor-mediated binding by directly labeled antibodies to reduce background staining in flow cytometry.
1. Incubate cells with Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ (≤1 µg per 1 x 10⁶ cells in 100 µl total staining volume) at 4°C for 5 minutes.
2. Add the conjugated primary antibody directly to the preincubated cells. Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ does not need to be washed away prior to cell staining.For specific conjugates of this clone, review literature for suggested application details.

Clone 2.4G2 recognizes the FcγIII and FcγII receptors.

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.

IgG

Low affinity receptors for IgG

1.) Titas, J. A. et al. (1982) J. Immunol. 133:556
2.) Rodewald, H. et al. (1992) Cell 69:139
3.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5 Journal Link