Anti-Mouse CD366 (Tim-3) – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Anti-Mouse CD366 (Tim-3) – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Product No.: T902
- -
- -
Clone RMT3-23 Target Tim-3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Havcr2 Isotype IgG2a κ Applications IHC FF , PhenoCycler® |
- -
- -
CODEX® DetailsValidation Notes Clone RMT2-23 has been validated for use on CODEX® using mouse T-cell Sarcoma fresh frozen (FF) tissue. Tissue Screened T-cell Sarcoma Tissue Preparation FF (Fresh Frozen) Antibody and Reporter DetailsReactivity Species Mouse Host Species Rat Concentration 0.5 mg/ml Immunogen This antibody was produced using recombinant mouse TIM-3 Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. Storage and Handling This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Applications and Recommended Usage? Quality Tested by Leinco CODEX® This CD366/Tim-3 (Clone RMT3-23) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml. RRID AB_2892983 Country of Origin USA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone RMT2-23 recognizes an epitope on mouse Tim-3. Antigen Distribution Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Background Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9. Antigen DetailsProtein Ligand/Receptor Putative ligand on resting CD4+ lymphocytes Function May play a role in the development of immune responses and the development of Th1-mediated responses PubMed NCBI Gene Bank ID References & Citations1. Monney, L. et al. (2002)Nature 415, 536–541. 2. Gao, X. et al. (2012) PLOS ONE 7, e30676. 3. Anderson, A. C. et al. (2007) Science 318, 1141–1143. 4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743. 5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304. 6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177. 7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101. 8. Zhu C, et al.. Nature Immunology. 2005;6:1245–1252. Technical Protocols PhenoCycler® |
Related Products
Formats Available
- -
- -
Prod No. | Description |
|---|---|
T902 | |
T700 | |
T710 | |
T730 | |
T750 | |
T740 | |
T720 | |
T715 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
