Anti-Mouse CD4 -(Clone GK1.5) Purified
Anti-Mouse CD4 -(Clone GK1.5) Purified
Product No.: C211
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Clone GK1.5 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4mut, L3T4, Ly-4 Isotype IgG2b κ Applications B , Costim , Depletion , FC , ICC , IHC FF , IP |
Data
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Mouse CTL clone V4 Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829192 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B Costim Depletion IHC IP ICC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1 Antigen Distribution The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. Ligand/Receptor MHC class II molecule PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Hendrickson WA et al. (1994) Structure 2: 59 Technical ProtocolsB Costim Depletion  ICC IHC FF  Certificate of Analysis |
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Formats Available
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Prod No. | Description |
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C1840 | |
C211 | |
C214 | |
C212 | |
C213 | |
C338 | |
C1640 | |
C1636 | |
C1333 | |
C1638 | |
C1637 | |
C2838 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
