Anti-Mouse CD48 (Clone HM48-1) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD48 (Clone HM48-1) – Purified in vivo GOLD™ Functional Grade

Product No.: C2380

[product_table name="All Top" skus="C2380"]

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Clone
HM48-1
Target
CD48
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BCM1, B-cell Membrane Protein, BLAST1
Isotype
IgG
Applications
B
,
FA
,
FC
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Recommended Dilution Buffer
Immunogen
Mouse T Lymphoma MBL-2
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this HM48-1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
B
IP
Costim
Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Armenian Hamster Anti-Mouse CD48 (Clone HM48-1) recognizes an epitope on Mouse CD48. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Ligand/Receptor
CD2, CD244
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

B
FA
Flow Cytometry
in vivo Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.