Anti-Mouse CD5 (Lyt-1) – Purified No Carrier Protein

Mouse thymus or spleen

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

53-7.3 activity is directed against mouse CD5 (Lyt-1).

CD5 (Lyt-1) is fundamental to immune homeostasis and immune tolerance, with many functions in T and B cells¹. CD5 acts as a modulator of T and B cell receptor signaling as well as a cell survival receptor in T and B cells. CD5 is also involved in thymocyte selection, T cell effector differentiation, induces regulatory T cells in the periphery, and modulates Th17 and Th2 differentiation as well as dendritic cell function. Because of its role as an immune checkpoint modulator, CD5 is a target of immunotherapy in the treatment of cancer, autoimmune disease, and infection.

53-7.3 was generated by immunizing LOU/Ws1/M rats with mouse (SJL/J or C3H/HeJ) spleen cells or thymocyte membranes². Spleen cells were subsequently fused with mouse myeloma NS-1 to produce a hybridoma. The antigen target CD5 (Lyt-1) was confirmed by comparative immunoprecipitation as well as blocking experiments using conventional alloantisera.

CD5 (Lyt-1) is a marker used to identify T cells, B1-a cells, B cell chronic lymphocytic leukemia cells, regulatory T cell (Tregs), regulatory B cells (IL-10 producing Bregs), and the earliest thymic progenitors. CD5 is also expressed on all lymphocytes except natural killer cells.

CD72, gp35-37

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