Anti-Mouse CD62L (Clone MEL-14)- Purified in vivo PLATINUM™ Functional Grade

C3H/eb mouse B lymphoma 38C-13

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

CD34, GlyCAM-1, MAdCAM-1

Lymphocyte homing to HEV, rolling on activated endothelium